What does MALDI/TOF stand for in laboratory testing?

MALDI/TOF stands for Matrix-assisted laser desorption/ionization Time-of-Flight. This technique is widely used in mass spectrometry for the analysis of biomolecules such as proteins and peptides. The MALDI process involves using a matrix to assist the desorption and ionization of the sample into the gas phase. The resulting ions are then accelerated in an electric field and travel down a time-of-flight tube. The time it takes for the ions to reach the detector is proportional to their mass-to-charge ratio, allowing for the determination of the molecular weights of the analytes.

The term "Matrix-assisted" reflects the role of the matrix in the process, which is crucial for preparing the sample and ensuring effective ionization. "Time-of-Flight" refers to the analysis method used to measure the time it takes for ions to travel from the ionization source to the detector.

This combination of techniques allows for high resolution and sensitivity in identifying and characterizing complex biological molecules, making MALDI/TOF a powerful tool in clinical laboratories and research settings.