What are the steps in preparing tissue samples for histology?

The correct answer outlines the comprehensive process essential for preparing tissue samples for histological examination. Each step plays a vital role in ensuring that the tissue sample can be accurately analyzed under a microscope.

Fixing is the initial step that preserves the tissue's structure and prevents decay. This process typically involves using a fixative solution, such as formalin, to stabilize the cellular components and make them amenable to further processing.

After fixation, grossing involves the examination and dissection of the tissue sample. In this step, the sample is carefully trimmed to the appropriate size and shape, focusing on areas of interest that should be examined further.

Embedding follows grossing, where the tissue is infiltrated with a medium, commonly paraffin wax, which provides support and allows for precise slicing into thin sections. This is crucial for achieving consistent, high-quality cuts that can be analyzed microscopically.

Cutting, or microtomy, involves slicing the embedded tissue into very thin sections, typically between 4-10 micrometers thick. These sections must be uniform to allow for accurate staining and evaluation.

Staining is the next step, where various dyes are applied to the tissue sections to enhance contrast and highlight specific cellular structures. Different stains can be used depending on the